Asterand Bioscience’s selection of immortalized cell lines will further your research in the mechanisms of cancer growth and progression. Our cell lines derive directly from freshly isolated solid tumors, including breast and prostate tissues as well as hematological samples. Each cell line purchased includes clinical data delivered with the cell aliquots. Our stringent quality control processes requires authentication including sterility testing, viability, and plating efficiency determined by lot. Immortalized cell lines are cryopreserved in 1 x 106 ampules.
Through licensing agreements with the University of Michigan and the Karmanos Cancer Institute, Asterand provides 11 well-characterized human breast cancer cell lines to assist in breast cancer research.
Ten SUM cell lines derived in Steve Ethier’s lab while present at the University of Michigan are available for purchase. Each cell line, from a single patient, represents a different subtype of breast cancer. All of the currently known oncogenes with altered expression patterns in breast cancer, in various combinations, are represented and characterized in the SUM lines. Furthermore, these cell lines have been described in over 50 peer-reviewed publications, describing isolation and growth conditions, gene expression data via Affymetrix GeneChips, and biomolecular markers, such as estrogen receptor and Her2 status. Click here to see a bibliography.
|CELL LINE NAME||Cell Source||ER||PR||ERBB-2 (HER2)||EGFR||Mutations||Growth Instructions|
|SUM-44PE||Human Breast||+||-||3, 4+||-||CS-04 (r03) SUM44PE Instructions for Use|
|SUM-52PE||Human Breast||-||-||3,4+||-||CS-05 (r03) SUM52PE Instructions for Use|
|SUM-102PT||Human Breast||-||-||3+||+||CS-06 (r03) SUM102PT Instructions for Use|
|SUM-149PT||Human Breast||-||-||(not activated)||2+||BRCA1||CS-07 (r03) SUM149PT Instructions for Use|
|SUM-159PT||Human Breast||-||-||-||+||CS-08 (r03) SUM159PT Instructions for Use|
|SUM-185PE||Human Breast||-||-||+||+||CS-09 (r03) SUM185PE Instructions for Use|
|SUM-190PT||Human Breast||-||-||3+||N/A||CS-10 (r03) SUM190PT Cell Line Instructions for Use|
|SUM-225CWN||Human Breast||-||-||3+||N/A||CS-12 (r03) SUM225CWN Instructions for Use|
|SUM-229PE*||Human Breast||-||-||-||2+||K-Ras||CS-13 (r03) SUM229PE Instructions for Use|
|SUM-1315MO2||Human Breast||-||-||+||+||CS-14 (r03) SUM1315MO2 Instructions for Use|
|*Unless otherwise cited all mutation data is referenced from the patient clinical data and refers to the tumor sample.|
Asterand offers three pairs of prostate tumor and normal cell lines. Researchers at the Karmanos Cancer Institute, Onyvax Ltd., and Asterand collaborated to derive these cell lines from tumors and adjacent “normal” tissues obtained at the time of prostatectomy. These prostate cell lines were immortalized via replication-defective retrovirus transferring the transforming HPV16 or HPV18 E6 and E7 genes.
**All prostate cell lines were immortalized were by exposing the cells to a replication-defective retrovirus transferring the transforming HPV16 or HPV18 E6 and E7 genes.
Through a licensing agreement with the Barbara Ann Karmanos Cancer Institute, Asterand provides well characterized human cell lines derived from patients with hematopoetic malignancies. These cell lines, developed by Doctors Ayad Al-Katib and Ramzi Mohammad, will be of interest to researchers seeking a cure for lymphoma, leukemia, pancreatic cancer, and macroglobulinema.