Venous blood is collected in a Beckton-Dickinson tube, inverted 6-10 times and allowed to sit for approximately 30 minutes. The tube(s) is centrifuged for 10-15 minutes. Serum or plasma is pipetted and aliquoted into 1.0 ml cryovials. Samples are frozen at minus 20°C or colder within 60 minutes of the blood draw and remain frozen until delivery to researcher. For serum, the standard blood collection tube for XB is 10.0 mL Becton-Dickinson (BD) Serum Separator Tube (SST). This tube is also referred to as a “tiger top” tube because of the striated red-gray colors that comprise the tube stopper. For plasma, the standard blood collection tube for XB is 10.0 mL Becton-Dickinson (BD) Ethylenediamine Tetraacetic Acid tube, (K2 EDTA). This tube is also referred to as a “purple top” because of the lavender color of the tube stopper.
PBMC isolation may be performed at the site or in the Asterand Bioscience lab. This is available through our custom procurement service and isolated according to the researcher’s instructions.
Whole blood is available through our custom procurement service. Venous blood is collected in a tube of the researcher’s choosing and processed according to the researcher’s instructions.
After blood is drawn, it sits at room temperature for 30 minutes prior to being placed into a centrifuge.
Asterand Bioscience recommends our collection sites spin blood for 10 minutes at 1,300 g. If a 1,300 g centrifuge is not available at the site samples may be spun for up to 15 minutes to ensure proper separation of the blood components. Blood is usually spun at ambient temperature unless otherwise requested. Some sites have limited capability to perform refrigerated centrifugation if required.
Asterand Bioscience requires that biofluid samples are stored by the collection site at -20°C or colder until they are shipped.
Serum, plasma, and whole blood may be shipped directly to the researcher from the collection site or shipped to Asterand Bioscience for long term storage. Samples sent directly to a researcher are generally delivered within 24 hours (domestic) and may be shipped with refrigerated/frozen chemical cold packs or dry ice. Samples sent to our facility are shipped on dry ice and stored at -80°C or colder.
The same tubes, processing, and freezing requirements are followed regardless of the subject’s disease status. However, blood from oncology subjects is generally drawn concurrent with pre-surgical laboratory tests which are likely to be a fasting draw. Normal subjects are identified during office visits at primary care clinics and may or may not be a fasting draw.
Our blood collection protocol does not specify whether the donor has fasted or not. Most oncology biofluid samples are pre-surgical blood draws performed concurrently for laboratory testing. The majority of our collection network performs the blood draw the morning of surgery.
Blood is drawn prior to anesthesia.
Hemolysis is performed by visual inspection of the sample only. This includes checking color against a BD Diagnostic “Relative Hemolysis Scale” color chart. Format, and volume of the biofluid samples are also checked. If a serum or plasma sample is red, it is quarantined and will not be available for a researcher.
The maximum draw is set by the IRB overseeing a specific protocol. Asterand Bioscience’s FPB (Tissue & Blood) Protocol states that a maximum of 20ml blood may be drawn pre-surgery. Normal control subjects and non-surgical diseased subjects may donate up to 50 ml blood. In all cases, the maximum allowed is at the discretion of the treating physician to ensure patient safety.