In Situ Hybridization (ISH) Service (RNAScope & ViewRNA)

Asterand Bioscience offers the latest generation branched-chain ISH technologies (ViewRNA and RNAscope), which represent a significant advance in sensitivity and throughput compared with traditional riboprobe ISH methods, for the detection and precise localization of cellular mRNA in human tissue. Ideally suited for target and biomarker validation and diagnostic assay development and testing, branched-chain ISH offers significant benefits, including:

Rapid and cost-effective, branched-chain ISH accelerates target and biomarker validation

Commercial availability of branched-chain ISH probes eliminates the need for additional up-front molecular biology work as would be required for traditional ISH.

 

Increased sensitivity reduces the risk of missing relevant expression patterns by enabling the detection of low-level mRNA production

Detecting extremely low levels of mRNA as compared with traditional ISH methodology, branched-chain ISH reduces the risk of false-negatives.

 

Highly-specific assays make use of FFPE tissue possible

Relying on the hybridization of large numbers of short oligonucleotide probe pairs, branched-chain ISH detects the less-well preserved mRNA in formalin-fixed, paraffin-embedded (FFPE) tissue as well as FFPE tissue microarrays (TMAs). Frozen sections can also be used and can offer greater sensitivity due to more complete RNA preservation.

ISH Multiplexing (ISH:ISH & ISH:IHC)

Via ISH:ISH multiplexing, Asterand Bio is able to localize the expression of multiple mRNA targets in the same tissue. Via ISH:IHC multiplexing, Asterand Bio is able to localize mRNA and protein expression in the same tissue.

ISH Optimization, Validation, and Sensitivity

Asterand Bio’s in situ hybridization protocols allow  for the precise localization of mRNA in human tissue. Utilizing customer-specific assay development, optimization and validation with either commercially available probes or custom target probes, our ISH offers additional levels of security in the data interpretation due to our assay optimization, validation of expression to know gene expression and high sensitivity detection capabilities.

IMG - RS/TBMV/ISH

in situ hybridization/ISH

High sensitivity in FFPE samples. Localization of GPR40 mRNA in a subset of islet cells within human pancreas. GPR40 measured by qRT-PCR at <1000 copies per 100ng total RNA. Pancreas was chosen because it presents particular challenges in the detection of low copy number mRNAs like GPR40 due to the high levels of endogenous RNAses.

 

For further information, or to discuss a specific study, contact us at advantage@asterandbio.com.