Asterand Bioscience offers the latest generation branched-chain ISH technologies (ViewRNA and RNAscope), which represent a significant advance in sensitivity and throughput compared with traditional riboprobe ISH methods, for the detection and precise localization of cellular mRNA in human tissue. Ideally suited for target and biomarker validation and diagnostic assay development and testing, branched-chain ISH offers significant benefits, including:
Commercial availability of branched-chain ISH probes eliminates the need for additional up-front molecular biology work as would be required for traditional ISH.
Detecting extremely low levels of mRNA as compared with traditional ISH methodology, branched-chain ISH reduces the risk of false-negatives.
Relying on the hybridization of large numbers of short oligonucleotide probe pairs, branched-chain ISH detects the less-well preserved mRNA in formalin-fixed, paraffin-embedded (FFPE) tissue as well as FFPE tissue microarrays (TMAs). Frozen sections can also be used and can offer greater sensitivity due to more complete RNA preservation.
Via ISH:ISH multiplexing, Asterand Bio is able to localize the expression of multiple mRNA targets in the same tissue. Via ISH:IHC multiplexing, Asterand Bio is able to localize mRNA and protein expression in the same tissue.
Asterand Bio’s in situ hybridization protocols allow for the precise localization of mRNA in human tissue. Utilizing customer-specific assay development, optimization and validation with either commercially available probes or custom target probes, our ISH offers additional levels of security in the data interpretation due to our assay optimization, validation of expression to know gene expression and high sensitivity detection capabilities.