With an extensive tissue bank, we specifically customize your immuno-oncology project to your needs. Asterand Bioscience has working protocols for targeting a wide range of oncology targets, plus routine immune cell markers.
Co-localization of CD3 and PD-1 in human tonsil, demonstrated in adjacent sections and dual immunohistochemistry in a single slide. The region highlighted in gold is part of the mantle zone of the germinal follicle in a tonsil (middle top and middle bottom panels).
Strong staining of CD3 T cells is seen, with a similar distribution to the strongly stained PD1 cells in the same region of the germinal follicle highlighted area (middle top and middle bottom panels).
Circles show follicle stained for CD20cy (green) but occasionally stained for CD3 (red) and PD-1 (purple). In the dual stained slide the immunostaining is still clear.
Human lung adenocarcinoma sample immunostained for T cells using anti CD3 (red) and B cells using anti-CD20cy (brown, yellow arrow). T cells and PD-1 were detected within the tumor itself (blue arrows) and also in the tumor stroma (green arrows). Localization of both CD3 and PD1 expressing cells appear to be closely matched in this sample. The inhibitory effect of PD-1 is accomplished through promoting apoptosis in antigen specific T-cells in lymph nodes and simultaneously reducing apoptosis in Treg cells (suppressor T cells).
Asterand Bioscience has also developed the ability to utilize triple immunostains with permanent chromogenic endpoints.
As a representative example, tonsil was stained with CD68, CD20cy and CD3 with different detection endpoints for the CD20cy and CD3, which clearly exemplify the importance of optimizing and choosing the best chromogenic stains based on expected cell densities within the sample.