Immuno-Oncology Expertise for Drug Discovery & Companion DX

 

With an extensive tissue bank, we specifically customize your immuno-oncology project to your needs. Asterand Bioscience has working protocols for targeting a wide range of oncology targets, plus routine immune cell markers.

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Co-localization of immune cells with targets cells

Co-localization of CD3 and PD-1 in human tonsil, demonstrated in adjacent sections and dual immunohistochemistry in a single slide. The region highlighted in gold is part of the mantle zone of the germinal follicle in a tonsil (middle top and middle bottom panels).

Strong staining of CD3 T cells is seen, with a similar distribution to the strongly stained PD1 cells in the same region of the germinal follicle highlighted area (middle top and middle bottom panels).

Circles show follicle stained for CD20cy (green) but occasionally stained for CD3 (red) and PD-1 (purple). In the dual stained slide the immunostaining is still clear.

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Human tonsil stained with CD20cy (upper left), CD3 (upper center), PD-1 (lower center), or dual stained for CD3/CD20cy (upper right). Negative control for all lower left and lower right.
 

Co-localization of immune cells with target cells in tumor tissue

Human lung adenocarcinoma sample  immunostained  for  T cells using  anti CD3 (red) and B cells using  anti-CD20cy (brown, yellow arrow). T cells  and PD-1 were detected within the tumor  itself (blue arrows) and  also in the tumor stroma (green arrows). Localization of both CD3 and PD1 expressing cells appear to be closely matched in this sample. The inhibitory effect of PD-1 is accomplished through promoting apoptosis in antigen specific T-cells in lymph nodes and simultaneously reducing apoptosis in Treg cells (suppressor T cells).

 

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Human lung adenocarcinoma dual stained with CD3/CD20cy (upper right) or PD-1 (upper left). Negative control for each in lower panels.

 

Triple Immunohistochemistry

Asterand Bioscience has also developed the ability to utilize triple immunostains with permanent chromogenic endpoints.

As a representative example, tonsil was stained with CD68, CD20cy and CD3 with different detection endpoints for the CD20cy and CD3, which clearly exemplify the importance of optimizing and choosing the best chromogenic stains based on expected cell densities within the sample.

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Upper left – Tonsil stained with CD68 (brown DAB), CD20cy (yellow) CD3 (purple). Upper right – Tonsil stained with CD68 (brown DAB), CD20cy (purple) and CD3 (yellow). Lower left – CD68 (brown DAB), CD20cy (red) and CD3 (purple). Lower right – CD68 (brown DAB), CD20cy (purple), CD3 (red).

 

For further information, contact us at advantage@asterandbio.com.